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osteogenesis assay kit  (Beyotime)


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    Beyotime osteogenesis assay kit
    Proliferation, osteogenic induction and mineralization of ROBs and RBMSCs induced by different types of Elec-exo. A) Schematic representation of the treatment of ROBs and RBMSCs with Norm-exo and Elec-exo (By Figdraw). B). C) Cell proliferation detected by CCK-8 assay, n = 5. D). E) Expression levels of genes associated with <t>osteogenesis.</t> (Bmp2, Col1A1, and Opn) in ROBs and RBMSCs detected by RT-qPCR on day 2, n = 3. F) ALP staining of ROBs (scale bar represents 100 μm) G) Corresponding quantitative analysis of integrated density values, n = 4. H) ARS staining after co-incubation for 21 days (scale bar represents 100 μm) I) Analysis of integrated density values, n = 4. Results are shown as mean ± SD. ns: non-significant differences, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.
    Osteogenesis Assay Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/osteogenesis assay kit/product/Beyotime
    Average 90 stars, based on 1 article reviews
    osteogenesis assay kit - by Bioz Stars, 2026-03
    90/100 stars

    Images

    1) Product Images from "Pretreated exosomes by electrical stimulation accelerate bone regeneration"

    Article Title: Pretreated exosomes by electrical stimulation accelerate bone regeneration

    Journal: Bioactive Materials

    doi: 10.1016/j.bioactmat.2025.04.019

    Proliferation, osteogenic induction and mineralization of ROBs and RBMSCs induced by different types of Elec-exo. A) Schematic representation of the treatment of ROBs and RBMSCs with Norm-exo and Elec-exo (By Figdraw). B). C) Cell proliferation detected by CCK-8 assay, n = 5. D). E) Expression levels of genes associated with osteogenesis. (Bmp2, Col1A1, and Opn) in ROBs and RBMSCs detected by RT-qPCR on day 2, n = 3. F) ALP staining of ROBs (scale bar represents 100 μm) G) Corresponding quantitative analysis of integrated density values, n = 4. H) ARS staining after co-incubation for 21 days (scale bar represents 100 μm) I) Analysis of integrated density values, n = 4. Results are shown as mean ± SD. ns: non-significant differences, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.
    Figure Legend Snippet: Proliferation, osteogenic induction and mineralization of ROBs and RBMSCs induced by different types of Elec-exo. A) Schematic representation of the treatment of ROBs and RBMSCs with Norm-exo and Elec-exo (By Figdraw). B). C) Cell proliferation detected by CCK-8 assay, n = 5. D). E) Expression levels of genes associated with osteogenesis. (Bmp2, Col1A1, and Opn) in ROBs and RBMSCs detected by RT-qPCR on day 2, n = 3. F) ALP staining of ROBs (scale bar represents 100 μm) G) Corresponding quantitative analysis of integrated density values, n = 4. H) ARS staining after co-incubation for 21 days (scale bar represents 100 μm) I) Analysis of integrated density values, n = 4. Results are shown as mean ± SD. ns: non-significant differences, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.

    Techniques Used: CCK-8 Assay, Expressing, Quantitative RT-PCR, Staining, Incubation



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    Image Search Results


    Proliferation, osteogenic induction and mineralization of ROBs and RBMSCs induced by different types of Elec-exo. A) Schematic representation of the treatment of ROBs and RBMSCs with Norm-exo and Elec-exo (By Figdraw). B). C) Cell proliferation detected by CCK-8 assay, n = 5. D). E) Expression levels of genes associated with osteogenesis. (Bmp2, Col1A1, and Opn) in ROBs and RBMSCs detected by RT-qPCR on day 2, n = 3. F) ALP staining of ROBs (scale bar represents 100 μm) G) Corresponding quantitative analysis of integrated density values, n = 4. H) ARS staining after co-incubation for 21 days (scale bar represents 100 μm) I) Analysis of integrated density values, n = 4. Results are shown as mean ± SD. ns: non-significant differences, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.

    Journal: Bioactive Materials

    Article Title: Pretreated exosomes by electrical stimulation accelerate bone regeneration

    doi: 10.1016/j.bioactmat.2025.04.019

    Figure Lengend Snippet: Proliferation, osteogenic induction and mineralization of ROBs and RBMSCs induced by different types of Elec-exo. A) Schematic representation of the treatment of ROBs and RBMSCs with Norm-exo and Elec-exo (By Figdraw). B). C) Cell proliferation detected by CCK-8 assay, n = 5. D). E) Expression levels of genes associated with osteogenesis. (Bmp2, Col1A1, and Opn) in ROBs and RBMSCs detected by RT-qPCR on day 2, n = 3. F) ALP staining of ROBs (scale bar represents 100 μm) G) Corresponding quantitative analysis of integrated density values, n = 4. H) ARS staining after co-incubation for 21 days (scale bar represents 100 μm) I) Analysis of integrated density values, n = 4. Results are shown as mean ± SD. ns: non-significant differences, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.

    Article Snippet: After 21 days of treatment, ROBs were stained for osteogenesis using the Osteogenesis Assay Kit (Beyotime, China).

    Techniques: CCK-8 Assay, Expressing, Quantitative RT-PCR, Staining, Incubation